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ret tyrosine kinase activity  (MedChemExpress)


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    MedChemExpress ret tyrosine kinase activity
    Pregnant <t>Ret</t> flox-V805A mice were injected daily with vehicle or a small-molecule Ret <t>tyrosine</t> <t>kinase</t> inhibitor, NA-PP1, beginning E16.5 daily for 3 days. Renal structure and N glom in offspring were analyzed. ( A ) PAS staining of P1 kidneys exposed to 50 mg/kg of NA-PP1 shows that kidneys are smaller than vehicle-exposed controls and have no tubular dilatation or hydronephrosis; images were obtained with a Zeiss M2 Bio microscope with 10× eyepiece and mechanical stage adjusted to a total of 33× magnification. Trichrome staining of P1 kidneys indicates no evidence of fibrosis (magnification, 25×). ( B ) N glom was reduced in mice with prenatal exposure to NA-PP1 (32.25 mg/kg, n = 13; 50 mg/kg, n = 33; or 62.5 mg/kg, n = 28) compared with vehicle-exposed controls ( n = 17). * P < 0.0001, 1-way ANOVA followed by Tukey’s test for multiple comparisons; all groups significantly different from vehicle. ( C ) NA-PP1–exposed mice show decreased UB branching and truncated UB tips. Whole-mount P1 kidneys from vehicle- or NA-PP1–exposed (50 mg/kg) pups were immunolabeled with antibody to calbindin, followed by optical clearing before image acquisition with a laser confocal microscope and 3-D reconstruction (100× magnification). ( D ) Two-week-old kidneys of mice with prenatal exposure to NA-PP1 (50 mg/kg) contain proximal tubules, thick ascending limbs, distal convoluted tubules, and glomerular and peritubular capillaries. Kidneys were stained with proximal tubule marker LTA (white) and distal tubule marker TSC (red), shown in top panel. Thick ascending limb is labeled with THP (red) and collecting ducts with DBA (green) shown in middle panel. Bottom panel shows capillaries and small veins labeled with endomucin (red). Laminin immunostaining (green) highlights tubular basement membrane (400× magnification). ( E ) Kidney/body weight ratio in NA-PP1–exposed (50 mg/kg) adult mice is significantly lower than age-matched vehicle-exposed controls. * P < 0.01, Welch’s t test, vehicle n = 7; NA-PP1, n = 23.
    Ret Tyrosine Kinase Activity, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ret tyrosine kinase activity/product/MedChemExpress
    Average 94 stars, based on 30 article reviews
    ret tyrosine kinase activity - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Low nephron endowment increases susceptibility to renal stress and chronic kidney disease"

    Article Title: Low nephron endowment increases susceptibility to renal stress and chronic kidney disease

    Journal: JCI Insight

    doi: 10.1172/jci.insight.161316

    Pregnant Ret flox-V805A mice were injected daily with vehicle or a small-molecule Ret tyrosine kinase inhibitor, NA-PP1, beginning E16.5 daily for 3 days. Renal structure and N glom in offspring were analyzed. ( A ) PAS staining of P1 kidneys exposed to 50 mg/kg of NA-PP1 shows that kidneys are smaller than vehicle-exposed controls and have no tubular dilatation or hydronephrosis; images were obtained with a Zeiss M2 Bio microscope with 10× eyepiece and mechanical stage adjusted to a total of 33× magnification. Trichrome staining of P1 kidneys indicates no evidence of fibrosis (magnification, 25×). ( B ) N glom was reduced in mice with prenatal exposure to NA-PP1 (32.25 mg/kg, n = 13; 50 mg/kg, n = 33; or 62.5 mg/kg, n = 28) compared with vehicle-exposed controls ( n = 17). * P < 0.0001, 1-way ANOVA followed by Tukey’s test for multiple comparisons; all groups significantly different from vehicle. ( C ) NA-PP1–exposed mice show decreased UB branching and truncated UB tips. Whole-mount P1 kidneys from vehicle- or NA-PP1–exposed (50 mg/kg) pups were immunolabeled with antibody to calbindin, followed by optical clearing before image acquisition with a laser confocal microscope and 3-D reconstruction (100× magnification). ( D ) Two-week-old kidneys of mice with prenatal exposure to NA-PP1 (50 mg/kg) contain proximal tubules, thick ascending limbs, distal convoluted tubules, and glomerular and peritubular capillaries. Kidneys were stained with proximal tubule marker LTA (white) and distal tubule marker TSC (red), shown in top panel. Thick ascending limb is labeled with THP (red) and collecting ducts with DBA (green) shown in middle panel. Bottom panel shows capillaries and small veins labeled with endomucin (red). Laminin immunostaining (green) highlights tubular basement membrane (400× magnification). ( E ) Kidney/body weight ratio in NA-PP1–exposed (50 mg/kg) adult mice is significantly lower than age-matched vehicle-exposed controls. * P < 0.01, Welch’s t test, vehicle n = 7; NA-PP1, n = 23.
    Figure Legend Snippet: Pregnant Ret flox-V805A mice were injected daily with vehicle or a small-molecule Ret tyrosine kinase inhibitor, NA-PP1, beginning E16.5 daily for 3 days. Renal structure and N glom in offspring were analyzed. ( A ) PAS staining of P1 kidneys exposed to 50 mg/kg of NA-PP1 shows that kidneys are smaller than vehicle-exposed controls and have no tubular dilatation or hydronephrosis; images were obtained with a Zeiss M2 Bio microscope with 10× eyepiece and mechanical stage adjusted to a total of 33× magnification. Trichrome staining of P1 kidneys indicates no evidence of fibrosis (magnification, 25×). ( B ) N glom was reduced in mice with prenatal exposure to NA-PP1 (32.25 mg/kg, n = 13; 50 mg/kg, n = 33; or 62.5 mg/kg, n = 28) compared with vehicle-exposed controls ( n = 17). * P < 0.0001, 1-way ANOVA followed by Tukey’s test for multiple comparisons; all groups significantly different from vehicle. ( C ) NA-PP1–exposed mice show decreased UB branching and truncated UB tips. Whole-mount P1 kidneys from vehicle- or NA-PP1–exposed (50 mg/kg) pups were immunolabeled with antibody to calbindin, followed by optical clearing before image acquisition with a laser confocal microscope and 3-D reconstruction (100× magnification). ( D ) Two-week-old kidneys of mice with prenatal exposure to NA-PP1 (50 mg/kg) contain proximal tubules, thick ascending limbs, distal convoluted tubules, and glomerular and peritubular capillaries. Kidneys were stained with proximal tubule marker LTA (white) and distal tubule marker TSC (red), shown in top panel. Thick ascending limb is labeled with THP (red) and collecting ducts with DBA (green) shown in middle panel. Bottom panel shows capillaries and small veins labeled with endomucin (red). Laminin immunostaining (green) highlights tubular basement membrane (400× magnification). ( E ) Kidney/body weight ratio in NA-PP1–exposed (50 mg/kg) adult mice is significantly lower than age-matched vehicle-exposed controls. * P < 0.01, Welch’s t test, vehicle n = 7; NA-PP1, n = 23.

    Techniques Used: Injection, Staining, Microscopy, Immunolabeling, Marker, Labeling, Immunostaining, Membrane



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    Pregnant <t>Ret</t> flox-V805A mice were injected daily with vehicle or a small-molecule Ret <t>tyrosine</t> <t>kinase</t> inhibitor, NA-PP1, beginning E16.5 daily for 3 days. Renal structure and N glom in offspring were analyzed. ( A ) PAS staining of P1 kidneys exposed to 50 mg/kg of NA-PP1 shows that kidneys are smaller than vehicle-exposed controls and have no tubular dilatation or hydronephrosis; images were obtained with a Zeiss M2 Bio microscope with 10× eyepiece and mechanical stage adjusted to a total of 33× magnification. Trichrome staining of P1 kidneys indicates no evidence of fibrosis (magnification, 25×). ( B ) N glom was reduced in mice with prenatal exposure to NA-PP1 (32.25 mg/kg, n = 13; 50 mg/kg, n = 33; or 62.5 mg/kg, n = 28) compared with vehicle-exposed controls ( n = 17). * P < 0.0001, 1-way ANOVA followed by Tukey’s test for multiple comparisons; all groups significantly different from vehicle. ( C ) NA-PP1–exposed mice show decreased UB branching and truncated UB tips. Whole-mount P1 kidneys from vehicle- or NA-PP1–exposed (50 mg/kg) pups were immunolabeled with antibody to calbindin, followed by optical clearing before image acquisition with a laser confocal microscope and 3-D reconstruction (100× magnification). ( D ) Two-week-old kidneys of mice with prenatal exposure to NA-PP1 (50 mg/kg) contain proximal tubules, thick ascending limbs, distal convoluted tubules, and glomerular and peritubular capillaries. Kidneys were stained with proximal tubule marker LTA (white) and distal tubule marker TSC (red), shown in top panel. Thick ascending limb is labeled with THP (red) and collecting ducts with DBA (green) shown in middle panel. Bottom panel shows capillaries and small veins labeled with endomucin (red). Laminin immunostaining (green) highlights tubular basement membrane (400× magnification). ( E ) Kidney/body weight ratio in NA-PP1–exposed (50 mg/kg) adult mice is significantly lower than age-matched vehicle-exposed controls. * P < 0.01, Welch’s t test, vehicle n = 7; NA-PP1, n = 23.
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    Pregnant <t>Ret</t> flox-V805A mice were injected daily with vehicle or a small-molecule Ret <t>tyrosine</t> <t>kinase</t> inhibitor, NA-PP1, beginning E16.5 daily for 3 days. Renal structure and N glom in offspring were analyzed. ( A ) PAS staining of P1 kidneys exposed to 50 mg/kg of NA-PP1 shows that kidneys are smaller than vehicle-exposed controls and have no tubular dilatation or hydronephrosis; images were obtained with a Zeiss M2 Bio microscope with 10× eyepiece and mechanical stage adjusted to a total of 33× magnification. Trichrome staining of P1 kidneys indicates no evidence of fibrosis (magnification, 25×). ( B ) N glom was reduced in mice with prenatal exposure to NA-PP1 (32.25 mg/kg, n = 13; 50 mg/kg, n = 33; or 62.5 mg/kg, n = 28) compared with vehicle-exposed controls ( n = 17). * P < 0.0001, 1-way ANOVA followed by Tukey’s test for multiple comparisons; all groups significantly different from vehicle. ( C ) NA-PP1–exposed mice show decreased UB branching and truncated UB tips. Whole-mount P1 kidneys from vehicle- or NA-PP1–exposed (50 mg/kg) pups were immunolabeled with antibody to calbindin, followed by optical clearing before image acquisition with a laser confocal microscope and 3-D reconstruction (100× magnification). ( D ) Two-week-old kidneys of mice with prenatal exposure to NA-PP1 (50 mg/kg) contain proximal tubules, thick ascending limbs, distal convoluted tubules, and glomerular and peritubular capillaries. Kidneys were stained with proximal tubule marker LTA (white) and distal tubule marker TSC (red), shown in top panel. Thick ascending limb is labeled with THP (red) and collecting ducts with DBA (green) shown in middle panel. Bottom panel shows capillaries and small veins labeled with endomucin (red). Laminin immunostaining (green) highlights tubular basement membrane (400× magnification). ( E ) Kidney/body weight ratio in NA-PP1–exposed (50 mg/kg) adult mice is significantly lower than age-matched vehicle-exposed controls. * P < 0.01, Welch’s t test, vehicle n = 7; NA-PP1, n = 23.
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    Image Search Results


    Pregnant Ret flox-V805A mice were injected daily with vehicle or a small-molecule Ret tyrosine kinase inhibitor, NA-PP1, beginning E16.5 daily for 3 days. Renal structure and N glom in offspring were analyzed. ( A ) PAS staining of P1 kidneys exposed to 50 mg/kg of NA-PP1 shows that kidneys are smaller than vehicle-exposed controls and have no tubular dilatation or hydronephrosis; images were obtained with a Zeiss M2 Bio microscope with 10× eyepiece and mechanical stage adjusted to a total of 33× magnification. Trichrome staining of P1 kidneys indicates no evidence of fibrosis (magnification, 25×). ( B ) N glom was reduced in mice with prenatal exposure to NA-PP1 (32.25 mg/kg, n = 13; 50 mg/kg, n = 33; or 62.5 mg/kg, n = 28) compared with vehicle-exposed controls ( n = 17). * P < 0.0001, 1-way ANOVA followed by Tukey’s test for multiple comparisons; all groups significantly different from vehicle. ( C ) NA-PP1–exposed mice show decreased UB branching and truncated UB tips. Whole-mount P1 kidneys from vehicle- or NA-PP1–exposed (50 mg/kg) pups were immunolabeled with antibody to calbindin, followed by optical clearing before image acquisition with a laser confocal microscope and 3-D reconstruction (100× magnification). ( D ) Two-week-old kidneys of mice with prenatal exposure to NA-PP1 (50 mg/kg) contain proximal tubules, thick ascending limbs, distal convoluted tubules, and glomerular and peritubular capillaries. Kidneys were stained with proximal tubule marker LTA (white) and distal tubule marker TSC (red), shown in top panel. Thick ascending limb is labeled with THP (red) and collecting ducts with DBA (green) shown in middle panel. Bottom panel shows capillaries and small veins labeled with endomucin (red). Laminin immunostaining (green) highlights tubular basement membrane (400× magnification). ( E ) Kidney/body weight ratio in NA-PP1–exposed (50 mg/kg) adult mice is significantly lower than age-matched vehicle-exposed controls. * P < 0.01, Welch’s t test, vehicle n = 7; NA-PP1, n = 23.

    Journal: JCI Insight

    Article Title: Low nephron endowment increases susceptibility to renal stress and chronic kidney disease

    doi: 10.1172/jci.insight.161316

    Figure Lengend Snippet: Pregnant Ret flox-V805A mice were injected daily with vehicle or a small-molecule Ret tyrosine kinase inhibitor, NA-PP1, beginning E16.5 daily for 3 days. Renal structure and N glom in offspring were analyzed. ( A ) PAS staining of P1 kidneys exposed to 50 mg/kg of NA-PP1 shows that kidneys are smaller than vehicle-exposed controls and have no tubular dilatation or hydronephrosis; images were obtained with a Zeiss M2 Bio microscope with 10× eyepiece and mechanical stage adjusted to a total of 33× magnification. Trichrome staining of P1 kidneys indicates no evidence of fibrosis (magnification, 25×). ( B ) N glom was reduced in mice with prenatal exposure to NA-PP1 (32.25 mg/kg, n = 13; 50 mg/kg, n = 33; or 62.5 mg/kg, n = 28) compared with vehicle-exposed controls ( n = 17). * P < 0.0001, 1-way ANOVA followed by Tukey’s test for multiple comparisons; all groups significantly different from vehicle. ( C ) NA-PP1–exposed mice show decreased UB branching and truncated UB tips. Whole-mount P1 kidneys from vehicle- or NA-PP1–exposed (50 mg/kg) pups were immunolabeled with antibody to calbindin, followed by optical clearing before image acquisition with a laser confocal microscope and 3-D reconstruction (100× magnification). ( D ) Two-week-old kidneys of mice with prenatal exposure to NA-PP1 (50 mg/kg) contain proximal tubules, thick ascending limbs, distal convoluted tubules, and glomerular and peritubular capillaries. Kidneys were stained with proximal tubule marker LTA (white) and distal tubule marker TSC (red), shown in top panel. Thick ascending limb is labeled with THP (red) and collecting ducts with DBA (green) shown in middle panel. Bottom panel shows capillaries and small veins labeled with endomucin (red). Laminin immunostaining (green) highlights tubular basement membrane (400× magnification). ( E ) Kidney/body weight ratio in NA-PP1–exposed (50 mg/kg) adult mice is significantly lower than age-matched vehicle-exposed controls. * P < 0.01, Welch’s t test, vehicle n = 7; NA-PP1, n = 23.

    Article Snippet: To inhibit Ret tyrosine kinase activity, pregnant female mice were injected i.p. with NA-PP1 (Medchem express, HY-13941/CS-1804) or vehicle (cremaphor/saline/ethanol in 1:7:2 ratio) from E16.5 through E18.5 at the dose of 32.25 mg/kg, 50 mg/kg, and 62.5 mg/kg, respectively.

    Techniques: Injection, Staining, Microscopy, Immunolabeling, Marker, Labeling, Immunostaining, Membrane